Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test
Identifieur interne : 000304 ( France/Analysis ); précédent : 000303; suivant : 000305Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test
Auteurs : J. J. Chomel [France] ; D. Thouvenot [France] ; M. Onno [France] ; C. Kaiser [France] ; J. M. Gourreau [France] ; M. Aymard [France]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1989.
English descriptors
- Teeft :
- Cell cultures, Clin, Clinical specimens, Elisa, Embryonated, Embryonated eggs, Haemagglutinin, Harmon, Immunocapture, Immunocapture elisa, Immunocapture elisa test, Immunofluorescence, Indirect immunofluorescence, Indirect immunofluorescence test, Influenza, Influenza diagnosis, Influenza infection, Microbial, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Nasal swabs, Nasopharyngeal, Nasopharyngeal secretions, Porcine, Porcine strains, Rabbit antiserum, Rapid diagnosis, Swab, Virus isolation.
Abstract
Abstract: An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.This test can be carried out in 3 h.
Url:
DOI: 10.1016/0166-0934(89)90102-X
Affiliations:
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A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.This test can be carried out in 3 h.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Chomel, J J" sort="Chomel, J J" uniqKey="Chomel J" first="J. J." last="Chomel">J. J. 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